Coding
ColOx

Part:BBa_K1313000:Design

Designed by: Eddie Cano   Group: iGEM14_ITESM-CEM   (2014-10-11)


Cholesterol Oxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1701
    Illegal AgeI site found at 520
    Illegal AgeI site found at 1390
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any design considerations.


Source

This part was synthesized, this originally belongs to Rhodococcus jostii RHA1 genome.

References

1. Lyons MA, Brown AJ. Molecules in focus: 7-ketocholesterol. Int J Biochem Cell Biol. 1999; 31: 369-375.
2. Mathieu JM, Mohn WW, Eltis LD, LeBlanc JC, Stewart GR, Dresen C, et al. 7-Ketocholesterol catabolism by Rhodococcus jostti RHA1. Appl Environ Microbiol. 2009 Oct 26; 76(1): 352-355.
3. Mathieu JM, Schloendron J, Rittmann BE, Álvarez PJJ. Microbial degradation of 7-ketocholesterol. Biodegradation. 2008; 19: 807-813.
4. Mathieu JM. Strategies for the mitigation of oxysterol-induced cytotoxicity. Texas: Rice University; 2011.